超声波辅助象拔蚌多糖提取及抗氧化活性研究

Study on Ultrasonic-assisted Extraction and Antioxidant Activity of Polysaccharides from Panope generosa

采用蒽酮比色法测定多糖含量。基于超声时间、温度、固液比单因素对象拔蚌多糖提取率影响的基础上,设计三因素三水平正交实验研究象拔蚌多糖最佳提取工艺。并测定象拔蚌多糖的总抗氧化活性和羟自由基清除活性。结果表明,影响多糖提取的首要因素是固液比,其次是超声时间和超声温度。象拔蚌多糖最佳提取条件为:温度60℃、超声时间30 min、固液比1:12。象拔蚌多糖总抗氧化活性和羟自由基清除活性均与浓度有明显的量效关系,表明超声辅助法是象拔蚌多糖提取的一种有效方法,高浓度象拔蚌多糖具有较强的抗氧化活性。

Ultrasonic method was employed to extract polysaceharides from Panope generosa. Effect of ultrasonic time, temperature and solid-liquid ratio on the extraction of polysaccharides from Panope generosa were exampled. Furthermore, orthogonal experiment was applied to obtain the optimum extraction condition. The concentration of polysaccharides from Panope generosa was determined by anthrone method. The total antioxidant activity and capability to scavenge hydroxyl radicals of the polysaccharides from Panope generosa were tested using commercial kits. The main factor affecting extracting rate of polysaccharides was solid-liquid ratio,followed by ultrasonic time and temperature. The optimum extraction conditions to obtain the highest polysaccharides were 60 ℃ ,30 min and solid-liquid ratio of 1： 12. Both the total antioxidant activity and scavenging capability of polysacchafides were obviously related to the concentrations. It indicated that ultrasonic method was a very effective method of polysaccharides extraction and the polysaccharides have good antioxidant activity at a high concentration.