二母颗粒对小鼠Lewis肺癌模型的肿瘤血管生成的影响

The effect of Ermu particles on tumor angiogenesis in Lewis lung carcinoma bearing mice

目的观察二母颗粒对C57BL/6小鼠体内Lewis肺癌生长、转移以及血管生成的影响及其作用机制。方法制备C57BL/6小鼠Lewis肺癌模型,随机分为二母颗粒高、中、低(3、2、1 g.kg-1生药)剂量组、环磷酰胺组、沙利度胺组、模型组。分别给予相应药物后,观察各组小鼠的肿瘤生长情况及肺转移发生率,采用免疫组化方法观察瘤内血管内皮细胞生长因子(VEGF)和微血管密度(MVD)的表达。结果二母颗粒高、中、低剂量组连续ig给药14 d,对小鼠Lewis肺癌的抑瘤率分别为30.80%、10.63%、0。其中,二母颗粒高剂量组的瘤重、抑瘤率、VEGF及MVD的表达与模型组比较均有显著差异(P<0.05)。结论二母颗粒具有抑制小鼠Lewis肺癌的作用,其作用机制可能与抑制瘤内VEGF和MVD的生成有关。

OBJECTIVE To observe the effect of Ermu particles on the growth,metastasis and angiogenesis of C57BL/6 Lewis lung carcinoma bearing mice,and explore the corresponding mechanism.METHODS Lewis lung carcinoma bearing model was established in C57BL/6 mice.The model mice were stochastically divided into high dose（3 g·kg-1） Ermukeli particles group,middle dose（2 g·kg-1） Ermu particles group,low dose（1 g·kg-1） Ermu particlesi group,cyclophosphamide group,thalidomide group and model group.The growth condition of tumor and the rate of pulmonary metastasis were measured,and then the expression of vascular endothelial growth fator（VEGF） and microvessel density（MVD） in tumor were investigated by immunohistochemical staining.RESULTS On the basis of high,middle,low doses Ermu particles group treated by gavage for 14 d,the inhibition rates toward tumor were 30.80%,10.63% and 0%,respectively.The high dose Ermu particles group was significantly different from model group in terms of tumor weight,inhibitory rate,expression of VEGF and MVD（P0.05）.CONCLUSION Ermu particles has the effect of inhibition toward C57BL/6 mice Lewis lung carcinomas,whose mechanism was related to inhibition toward generation of VEGF and MVD.