摘要
目的:观察炎性细胞因子IL-1β作用于SD大鼠髁突软骨细胞后,细胞内源性生长因子TGF-β表达的改变。方法:体外分离培养SD大鼠髁突软骨细胞,给予5ng/mL、10 ng/mL及50ng/mL浓度的IL-1β刺激,应用免疫组织化学技术检测刺激前后TGF-β相对含量,初步了解在IL-1β刺激下软骨细胞分泌表达情况的改变。结果:本研究中采用了0.2%Ⅱ型胶原酶和0.25%胰蛋白酶两种酶进行消化,成功地培养出髁突软骨细胞。髁突软骨细胞的细胞生长潜伏期较长,接种后48h至120h为对数生长期,120h后进入生长停滞期。随着培养时间的延长,第3代细胞生物学性状相对稳定,适合软骨组织工程研究之用;软骨细胞在IL-1β作用下,TGF-β表达增加。结论:72h内随着IL-1β浓度的增加,髁突软骨细胞内的TGF-β合成增加。
Objective：Intedeukin （IL）- 1βis a major catholic cytokine thai plays a pivotal role in cartilage destruciion. This study aims at finding out the response of rat condylar chondrocytes when IL- 113 stimulations are given. Methods： Condylar chondrocytes are separated from the mandibular condyle of SD rats, and cultured. Stimulations of IL - 1β at 5ng/mL lOng/mL and 50ng/mL were given to treat the cell. The expression of TGF - βwere detected by means of immunohistochemisty. Results： The study showed that ： Chondrocytes stimulated with IL - 1β showed increased expression of TGF - β in 72h. Conclusions： Within the sc;ope of this study, it is concluded that IL -1β incmases expression of TGF -β of rat condylar chondrocytes,
出处
《激光杂志》
CAS
CSCD
北大核心
2012年第6期73-74,共2页
Laser Journal
