银-组氨酸配合物-蒙脱石抗菌剂的制备及抗菌活性

Synthesis and Antibacterial Activity of Silver-histidine Complex Doped Montmorillonite Antibacterial Agent

以银-组氨酸配位阳离子[Ag(His)]+为前驱体,采用水热离子交换法制备了抗变色耐盐性的新型银-组氨酸配合物-蒙脱石抗菌剂(SHMMT).用电感耦合等离子体发射光谱仪(ICP)测定了抗菌剂的载银量,用X射线衍射仪(XRD)和热重-差热分析仪(TG-DTA)表征了抗菌剂的结构,用平板计数法测试了SHMMT对交替假单胞菌(Pseudoalteromonas carrageenovora)的抗菌活性,通过海水浸泡实验对抗菌剂的耐盐性和缓释性进行了测试.结果表明,当组氨酸与Ag+的摩尔比为1∶1时,SHMMT抗菌剂的载银量高达1.7 mmol/g,远大于蒙脱石(Na-MMT)的阳离子交换容量.该产品为白色粉末,具有很强的抗变色能力和抗菌活性.SHMMT在海水中浸泡45 d后杀菌率仍达80%,耐盐性和缓释性良好,是一种用途广泛的环保型抗菌材料.

Abstract A novel colorless salt-tolerant silver-histidine complex doped montmorillonite antibacterial agent（SHMMT） powder was synthesized by hydrothermal ion exchange reaction using silver-histidine complex ion [Ag （his）] ^＋ as precursor. The product was characterized by inductively coupled plasma spectrometer （ICP）, powder X-ray diffractometer （ XRD ） and thermogravimetric-differential temperature analyser （ TGDTA）. The antibacterial activity of SHMMT against Pseudoalteromonas carrageenovora was examined by a modified broth dilution test and the plate counting method. The salt-tolerant and slow-releasing properties of SHMMT were tested by sea water soaking method. The results show that the amount of Ag loaded on SHMMT powder reaches 1.7 mmol/g when the molar ratio of silver to histidine equals 1 ： 1, which is much more than the cationic exchange capacity（cec） of Na-MMT. SHMMT powder is pile white and has high bacterial activity. After being soaked in the sea water for 45 d, the 80%. bactericidal rate of the SHMMT powder still reaches 80%.