保护素D1对小鼠急性肺损伤的保护作用

Protective effect of PDI on mice with acute lung injury

目的研究保护素D1（PD1）对脂多糖（LPS）诱导的小鼠急性肺损伤的影响。方法40只雄性BABL／C小鼠随机分为正常对照组（Ns组）、急性肺损伤组（LPS组）、PD1组（P组）、PD1预先给药组（PL组）4组，每组10只。P组和PL组尾静脉给予200ng的PD1预处理，NS组和LPS组则给予等体积的生理盐水，30min后LPS组和PL组以3mg／kg的LPS气管内滴入，Ns组和P组则给予等量的生理盐水。气管内滴入脂多糖24h后，测定肺组织湿／干质量比（W／D）、髓过氧化物酶（MPO）活性，支气管肺泡灌洗液（BALF）中自细胞计数、蛋白含量及TNF-α、IL-10的水平，HE染色光镜下观察肺组织病理学变化。结果与LPS组相比，PL组肺组织病理学改变明显减轻，且W／D、MPO活性（U／g）、BALF中自细胞总数（10^4／mL）、蛋白含量（g/L）、TNF-α浓度（pg／mL）均明显降低（均P〈0．05），BALF中IL-10浓度（pg／mL）显著升高（P〈0．01）。结论PD1可以减轻脂多糖诱导的小鼠急性肺损伤。

Objective To study protective effects of Protectin D1 on acute lung injury induced by lipopolysaccharide in mice. Methods Forty male BABL/C mice were randomly divided into four groups（n = 10） ：control group （NS group）, acute lung injury group （LPS group）, PD1 ＋ LPS group （PL group）, PD1 group（P group）. P group and PL group were pretreated by PD1 via caudal vein 30 min prior to LPS administration, NS group and LPS group were given the same volume of saline. Intratracheal injection of LPS with dosage of 3 mg/kg for establishing the model of ALI mice , equal amount of saline were intratracheally injected to the control group. At 24 h after LPS administration, lung wet/dry weight ratio （ W/D）, myeloperoxidase （MPO） activity, the number of leukocytes, protein content and TNF - ct and IL - 10 levels in bronchoalveolar lavage fluid （BALF） were determined. In addition, part of lung tissue was detected by morphological analysis. Results Compared to LPS group, the histological changes of lung injury were lessened by PD1 ; lung MPO activity, W/D, the number of leukocytes, total protein content, TNF - α levels in BALF were all decreased in PL group （ all P 〈 0.05） ; However, IL - 10 levels in BALF was raised（P 〈0.01 ）. Conclusion Protectin D1 can reduce lipopolysaccharide -induced acute lung injury in mice.