微波法提取紫苏黄酮类物质及其成分分析

Microwave-Assisted Extraction and Composition Analysis of Flavonoids from Perilla frutescens Leaves

研究微波法提取紫苏黄酮类物质的最佳工艺条件。采用单因素及响应面优化试验,分别考察紫苏粒径、提取时间、微波温度、乙醇体积分数及料液比对提取率的影响。结果表明:紫苏粒径20目、提取时间70min、温度71℃、乙醇体积分数61%、料液比1:20(g/mL)时,黄酮得率最高为59.28mg/g。紫苏提取液经柱层析法纯化后,经全波长扫描和高效液相色谱显示,该物质主要成分为芦丁、木犀草素等。

Response surface methodology was used to optimize conditions for the microwave-assisted extraction of flavonoids from Perilla frutescens leaves.The effects of raw material particle size,extraction time,temperature,ethanol concentration and solid-to-solvent ratio on extraction efficiency were investigated.The optimal conditions for extracting flavonoids from Perilla frutescens leaves were found to be extraction at 71 ℃ for 70 min with 61% ethanol at a solid-to-solvent ratio of 1：20（g/mL） and a raw material particle size of 20 mesh.Under these conditions,the maximum extraction yield of flavonoids was 59.28 mg/g.The obtained extract was purified by D152 macroporous resin column chromatography and the purified product mainly consisted of lutin and luteolin as demonstrated by UV absorption spectrometry and HPLC.