大麦芽根蛋白提取工艺条件优化

Optimization of Extraction Conditions for Protein from Barley Malt Roots

为充分利用大麦芽生产的副产物——大麦芽根中的蛋白资源,采用国家标准方法测定麦芽根的主要成分,通过正交试验研究碱法提取麦芽根蛋白的最佳工艺条件。结果表明:麦芽根的蛋白质、灰分、水分、脂肪及粗纤维质量分数分别为29.2%、6.9%、6.42%、1.4%和10.7%。正交试验结果表明,麦芽根中蛋白提取的最佳工艺条件为浸提液pH9.0、料液比1:25(g/mL)、浸提时间60min、浸提温度40℃,在此条件下麦芽根蛋白质的浸提率为63.5%。该工艺提取麦芽根蛋白简便且提取率高。

Barley malt roots as a byproduct of malt product was analyzed for proximate chemical composition according to the national standards.Meanwhile,the extraction of alkali-soluble protein from barley malt roots was optimized by orthogonal array design method.Barley malt roots contained 29.2% protein,6.9% ash,6.42% water,1.4% fat and 10.7% crude fiber.The optimal conditions for protein extraction were found to be extraction at 40 ℃ and pH 9.0 for 60 min with a solid-to-solvent ratio of 1：25（g/mL）,resulting in a protein yield of 63.5%.The optimized procedure provided a simple and high-yield method for protein extraction from barley malt roots.