超声波微波协同提取海蒿子多糖工艺

Optimization of Ultrasonic-Microwave Assisted Extraction of Polysaccharides from Sargassum pallidum

目的:建立海蒿子多糖超声波-微波协同提取工艺,提高海蒿子多糖的提取率。方法:利用硫酸-苯酚法考察正交试验在不同粒度、提取时间、料液比、超声波功率、微波功率条件下多糖提取率,选出最佳超声波-微波协同提取工艺。结果:最佳提取工艺为粒度40目、提取时间20min、料液比1:25(g/mL)、超声波功率75%、微波功率200W,其中粒度对多糖提取率的影响最大。结论:超声波-微波协同提取能缩短提取时间并提高海蒿子多糖提取率。

A high-yield method for the extraction of polysaccharides from Sargassum pallidum was proposed using an integrated ultrasonic and microwave reaction system.The extraction yield of polysaccharide was measured by phenol-sulferic acid method.The optimal conditions of raw material particle size,extraction time,solid-to-solvent ratio,ultrasonic power and microwave power for extracting polysaccharides from Sargassum pallidum were 40 mesh,20 min,1：25（g/mL）,75% and 200 W,respectively,as determined by orthogonal array design.Among five operating parameters,raw material particle size was the most important factor for polysaccharide extraction.The combined use of ultrasonic and microwave provided a time-saving and high-yield method for polysaccharide extraction from Sargassum pallidum.