西瓜蜂花粉β-胡萝卜素超临界萃取工艺优化和抗氧化活性研究

Optimization of Supercritical Fluid Extraction and Antioxidant Activity of Carotenoids from Watermelon Bee Pollen

利用响应面法对西瓜蜂花粉β-胡萝卜素超临界二氧化碳萃取条件优化,并对其进行抗氧化活性评价。结果发现,最佳萃取条件为萃取温度51℃、萃取压力34MPa、萃取时间80min,在此条件下β-胡萝卜素的提取率达到8.79mg/100g;抗氧化活性研究结果表明,与Trolox、β-胡萝卜素标准品相比,萃取物具有较高的清除ABTS+能力,而其总还原力和对DPPH自由基的清除能力则强于β-胡萝卜素而弱于Trolox。

Response surface methodology was used to optimize conditions for the supercritical fluid extraction with carbon dioxide of β-carotene from watermelon bee pollen, and crude β-carotene extract was tested for antioxidant activity. The optimal conditions for β-carotene extraction were found to be extraction at 51℃ and 34 MPa for 80 min. Under these conditions, the extraction yield of β-carotene was 8.79 mg/100 g. The resulting extract had stronger scavenging activity against ABTS free radials than Trolox and fl-carotene standard. Moreover, its total reducing power and DPPH radical scavenging activity were higher than those offl-carotene standard but weaker than those of Trolox.