产黑色素海洋放线菌的分离、鉴定和产色素条件优化

Screening,identification of marine actinomycete strain HT-18 and its optimization of melanin-producing conditions

【目的】从连云港高公岛沿岸海底泥样中分离筛选到一株产黑色素的海洋放线菌。【方法】对筛选得到的菌株HT-18所产的色素进行紫外-可见吸收光谱、红外光谱分析。通过形态特征、培养特征、生理生化测定以及16S rRNA序列的系统发育学分析确定菌株HT-18的分类学地位。采用单因素对产色素发酵条件进行优化。【结果】菌株HT-18所产色素为黑色素。鉴定该菌株属链霉菌属(Streptomyces)。最佳产色素条件为:初始pH7.0,装液量为70/250 mL,温度31°C,发酵时间为3 d。【结论】海洋链霉菌HT-18是一株具有研究和应用潜力的产黑色素菌株。

[Objective] A melanin-producing actinomycete was isolated from the mud collected from Gaogong Island, Lianyungang. [Methods] The pigment seperated from the fermentation broth of HT-18 was characterized by UV-visible absorption spectroscopy and infrared spectroscopy. The strain was clarified by colonial morphology, physiological and biochemical tests and 16S rRNA sequence phylogenetic analysis. The shake flask condition for pigment fermentation was optimized using single factor test. [Results] The results showed that HT-18’s production was melanin. The strain was preliminary identified Streptomyces. The optimal fermentation process was that 70 mL of tyrosine medium was contained in a 250 mL flask for 3 d fermentation at initial pH 7.0, 31 °C. [Conclusion] HT-18 was a melanin-producing strain with research and application potential.