摘要
目的研究九香虫三氯甲烷浸提物对胃癌细胞SGC-7901和肝癌细胞HepG2细胞增殖和细胞周期的影响。方法本研究采用冷浸法获得九香虫的三氯甲烷浸提物;MTT法分析三氯甲烷浸提物对两种癌细胞体外增殖的抑制作用;流式细胞术分析三氯甲烷浸提物对两种癌细胞的细胞周期的影响。结果九香虫三氯甲烷浸提物能抑制SGC-7901细胞和HepG2细胞的体外增殖并呈明显的剂量依赖性,IC50分别为1 193.52和964.34μg/mL;流式细胞术分析表明,三氯甲烷浸提物作用后HepG2细胞的S期和G2/M期细胞比例明显降低,G0/G1期细胞比例明显升高。结论九香虫三氯甲烷浸提物能抑制两种肿瘤细胞的体外增殖,对HepG2细胞抗肿瘤活性可能是由于通过其细胞周期的阻滞引起。
AIM To study the effects of crude extracts of Aspongopus chinensis Dallas on cell proliferation and cell cycle of SGC-7901 and HepG2 cell lines.METHODS The crude extracts were extracted by chloroform infusion.The antiproliferative effects of crude extracts were assessed by MTT assays.The cell cycle was measured by flow cytometry.RESULTS The crude extracts could inhibit the proliferation of SGC-7901 and HepG2 cell lines in a dose-dependent manner.SGC-7901 and HepG2 cell lines were sensitive to crude extracts with an IC50 of 1 193.52 and 964.34 μg/mL,respectively.From the result of flow cytometry analysis,crude extracts,as compared with the untreated cells,caused significant reduction in cell ratio at the S and G2/M phases,and an increase in cell ratio at the G0/G1 phases.CONCLUSION The crude extracts could inhibit proliferation of both cancer cell in vitro,and especially arrest human liver cancer cells at the G0/G1 phase of the cell cycle for HepG2.
出处
《中成药》
CAS
CSCD
北大核心
2012年第12期2278-2281,共4页
Chinese Traditional Patent Medicine
基金
贵州省中药现代化项目(黔科合中药字【2012】5001-1号)
贵州省中医药管理局项目(QZYY2011-69)
