重庆后山栀子的UPLC-PDA指纹图谱研究

UPLC-PDA fingerprint of Gardeniae Fructus from Houshan District,Chongqing

目的建立重庆后山地区栀子的UPLC指纹图谱。方法采用UPLC-PDA结合可变波长法,Waters ACQUITYUPLC BEH C18(50 mm×2.1 mm,1.7μm)色谱柱,流动相为甲醇-0.2%磷酸水溶液,梯度洗脱,检测波长为238和440 nm,体积流量0.3 mL/min,柱温30℃。并结合化学统计学方法 (相似度和主成分分析)对指纹图谱进行评价分析。结果建立了重庆后山栀子的UPLC指纹图谱,标定了18个共有峰,并用对照品指认其中2个峰(栀子苷、西红花苷-I);25批样品的相似度均大于0.95,重庆后山栀子与其他产地栀子药材样品间相似度结果差异较小。结论从相似度和主成分分析结果表明重庆后山栀子和市售栀子之间具有较高的一致性。

AIM To investigate UPLC fingerprint of Gardeniae Fructus cultivated in Houshan District,Chongqing.METHODS A method of ultra-performance liquid chromatography with photodiode array detector（UPLC-PDA） was developed for chemical fingerprint analysis of Gardeniae Fructus,and the samples were separated on a Waters ACQUITY UPLC BEH C18（50 mm×2.1 mm,1.7 μm） column with column temperature of 30 ℃ eluted with methanol and water containing 0.02% phosphate acid as mobile phases in a linear gradient mode.Flow rate was set at 0.3 mL/min and the detection wavelengths were set at 238 nm and 440 nm.The chemometrics procedures,including similarity analysis（SA） and principal component analysis（PCA）,were applied to classifying.RESULTS Eighteen co-peaks in the UPLC fingerprint of Gardeniae Fructus were established,and 2 peaks were identified as geniposide and crocin-I among them.All of the similarities were greater than 0.95,and there were no significant differences.CONCLUSION The results obtained from SA and PCA show a good consistence between the cultivated and the commercial Gardeniae Fructus.