摘要
目的:探讨山东地区汉族人群13号染色体上与冠心病易感性相关联的遗传位点。方法:采集经冠状动脉造影确诊的冠心病患者血液标本156例、对照组1 000例,在13号染色体上选取间隔为10 cm遗传距离的14个微卫星遗传标记,利用DNA混合池技术分别对两组样本进行基因组扫描,采用CLUMP软件对比两组间各位点等位基因频率的差异,分析13号染色体上冠心病的易感基因位点。结果:在D13S263(13q14.11)和D13S156(13q22.1)位点,冠心病组与对照组的等位基因频率差异有统计学意义(P<0.05)。结论:山东地区汉族人群13号染色体13q14.11及13q22.1区域中存在与冠心病易感性的关联,冠心病致病基因或调控因子可能位于其附近。
Objective ：To explore the relationship between chromosome 13 and coronary artery disease（CAD） in patients of Han nationa lity in Shandong area. Methods：Our work included 2 groups, CAD group,n= 156 and Control group, n = 1000 healthy volunteers. A total of 14 microsateUite markers on chromosome 13 spaced at approximate 10 cm were screened in 2 separate DNA pools for 2 groups. CLUMP software was performed to calculate and compare the differences in allele frequency of each locus between 2 groups. Results ：Statistic differences of allele frequencies were found at D13S263 （13q14. 11 ）and D13S156 （13q22. 1 ）loci between CAD group and Control group,P〈 0. 05. Conclusion： Chromosome 13 loci 13q14. 11 and 13q22. 1 may contain CAD related gene or CAD related regulating factor in Han nationality in Shandong area of China.
出处
《中国循环杂志》
CSCD
北大核心
2012年第6期427-430,共4页
Chinese Circulation Journal
基金
山东省自然科学基金项目(2009ZRA01110)
