氯化镉和敌敌畏突发胁迫下斑马鱼的行为差异

Behavioral Differences of Zebrafish under Sudden Stress of Dichlorvos and Cadmium Chloride

采用计算机摄像跟踪技术,以正常水体中斑马鱼的行为变化为基础,比较突发性氯化镉和敌敌畏污染胁迫下斑马鱼的个体行为(游动速度、深度、转弯次数和加速度)和鱼群行为(平均距离和分散度)的变化规律。研究结果表明:斑马鱼在两种污染物突发性胁迫下,行为反应快速且敏感,游动行为响应与污染物种类和暴露时间直接相关;两种不同类型的污染胁迫下,斑马鱼的行为变化趋势基本符合环境压力模型,但其响应方式存在差异。氯化铬突发性胁迫下,斑马鱼有明显的过度活跃行为现象,游动深度显著下降,而敌敌畏突发性胁迫下,行为强度直接降低,鱼群聚集且停留在水面附近。斑马鱼的行为响应敏感、迅速,是实现在线水质监测的有效手段。

Based on the cadmium chloride on the depth, turning frequency behavior response of zebraflsh, the effects behavioral responses of zebrafish, including and acceleration） and the endpoints of frsh of sudden exposure to dichlorvos and individual endpoints （swimming speed, school （distance and dispersion）, were investigated by a system with computer-imaging technique. Results showed that zebrafish were sensitive to sudden exposure of CdC12 and DDVP, expressed by the quickly behavioral responses of zebraftsh. The behaviors of zebrafish depended on the kinds of chemicals and exposure time. The stress behavioral responses were in accordance with the stepwise stress model, however, the responses were changed with the different chemicals. Sudden exposure to CdCI2, fish was hyperactivity （increased swimming speed and turning frequency） and their swimming depth significantly decreased. When sudden exposure to DDVP, fish gathered with decrease swimming activity and stayed near the water surface. The behavior responses of zebrat＇tsh under envirottment stress could be available to monitor the accidental pollution.