毒死蜱对雄性小鼠生殖毒性的影响

Effect of Chlorpyrifos on Reproductive Toxicity of Male Mice

为研究毒死蜱对雄性小鼠生殖毒性的影响,利用不同浓度毒死蜱染毒小鼠。以昆明小鼠为受试动物,毒死蜱按3、6和12mg·kg-13个剂量水平,灌胃染毒小鼠7d。以睾丸组织匀浆测定活性氧(reactive oxygen species,ROS)和还原型谷胱甘肽(glutathione,GSH)的含量;以睾丸细胞测定DNA-蛋白质交联(DNA-protein crosslink,DPC)系数;并制作睾丸组织切片观察其病理损伤。实验结果表明:随着毒死蜱染毒剂量的升高,毒死蜱对小鼠睾丸的病理损伤逐渐加重;ROS含量和DPC系数逐渐上升,GSH含量逐渐降低,各指标呈一定的剂量-效应关系。染毒剂量≥6mg·kg-1时,GSH有显著差异(p<0.05),ROS有极显著差异(p<0.01),睾丸曲细精管的腔面增大,精子和生成精子的相关细胞明显减少;染毒剂量≥12mg·kg-1时,DPC有显著差异(p<0.05),ROS和GSH有极显著差异(p<0.01),睾丸曲细精管内,伸长型精子细胞和成熟精子消失,可见凋亡的生精细胞,间质细胞增生。以上结果表明,较高剂量(≥6mg·kg-1)的毒死蜱能导致小鼠睾丸的氧化损伤和病理损伤,产生生殖毒性。

To research the effect of reproductive toxicity of chlorpyrifos on male mice which were gavage with different concerntrations ofchlorpyrifos. Male mice were orally administrated with chlorpyrifos daily for 7 days at the concentrations of 3, 6 and 12 mg·kg-1, respectively. Afterwards, the contents of ROS （reactive oxygen species） and GSH （glutathione） in the testis tissue homogenates, and DPC （DNA - protein crosslink） coefficients in the testis cells were measured. Testis tissue sections were made then stained for pathological observations under microscope. Results showed that the contents of ROS and DPC coefficients gradually increased in a dose - dependent manner, whereas GSH content decreased. In the exposure group, with the dose of 6 mg·kg-1, GSH content was significantly higher than that of the control group （p 〈 0.05）;ROS contents had extremely significant differences （p 〈0.01）. Stained sections presented the increased cavity surface in the testicular seminiferous tubules. Sperm and sperm generation associated cells were obviously reduced. In the higher dose groups （12 mg·kg-1）, the differences ofDPC, ROS, and GSH were all significant （p 〈 0.01）. Within the testicular seminiferous tubules, elongations of sperm cells were blocked; mature sperms were hardly found;spermatogenic cells apoptosis and stromal cell proliferation were observed. It can be concluded that chlorpyri- fos at higher doses（≥6 mg·kg-1） can increase the oxidative stress and pathological damagein male mouse testis, which induced the reproductive toxicity .