复色2号方对鼠黑素瘤B16细胞酪氨酸酶的影响

Influence of Fuse-2 Recipe on Mice B16 Melanoma Cells Tyrosinase

目的:探讨复色2号方治疗白癜风的分子学作用机制。方法:采用MTT法测定复色2号方对B16细胞增值的影响,在酶标仪下测定并记录B16细胞增殖情况;采用酪氨酸酶多巴速率氧化法测定复色2号方对酪氨酸酶活性的影响;采用NaOH裂解法测定B16细胞黑素含量。结果:复色2号方药物浓度在1～1000g/L对黑素瘤B16细胞增殖无统计学意义(P>0.05),但对酪氨酸酶活性和黑素合成有统计学意义(P<0.05),呈浓度依赖性。结论:复色2号方对黑素瘤B16细胞增殖无影响,但对酪氨酸酶活性和黑素合成有较强的剂量相关性促进作用,可以达到治疗白癜风的目的。

Objective ： To explore molecular mechanism of fuse - 2 recipe on treating vitiligo. Methods ： To determine the influence of fuse- 2 recipe on B16 cell proliferation by MTT method, measuring and recording B16 cell proliferation by the microplate reader. To determine the influence of fuse - 2 recipe on B16 cell tyrosine~ activity by tyrosinase DOPA rate oxidation method. To determine the melanin content of B16 cell by NaOH decomposition method. Results ：There was no statistical significance（ P 〉 0.05 ） of fuse - 2 drug concentration at 1 ～1000g/L on B16 cell proliferation, but there was statistical significance（P 〈 0.05）on tyrosinase activity and melanin synthesis in a concentration dependent manner. Con- clusion： Without effect on B16 melanoma cell proliferation, fuse- 2 has a strong correlation effect during the dose on ty- rosinase activity and melanin synthesis ,which could achieve the goal of the treating vitiligo.