黄精多糖对脂多糖(LPS)诱导人脐静脉内皮细胞(HUVEC)损伤的保护机制研究

Research of Polygonatum Polysaccharide on the Protective Mechanism of LPS-Induced HUVEC Injury

目的:探讨黄精多糖对LPS诱导HUVEC损伤的保护作用。方法:采用HUVEC为研究对象,MTT法检测黄精多糖对LPS诱导HUVEC损伤的影响,Hoechst33258荧光染色法检测黄精多糖抗凋亡的作用效果。结果:黄精多糖与HUVEC共培养后没有引起细胞活力的明显改变,排除了实验中药物的直接细胞毒作用,通过MTT法可以发现,黄精多糖可以显著减少LPS与HUVEC共培养后,LPS对HUVEC造成的损伤(P<0.05);通过Hoechst33258荧光染色发现正常组细胞核较大且染色均匀,LPS与内皮细胞共培养后,核内可见浓染致密的颗粒状荧光,呈现出核固缩和核碎裂特征,给予黄精多糖后有所改善(P<0.05)。结论:黄精多糖具有保护LPS诱导HUVEC损伤的作用,其机制是否通过抗凋亡的途径实现有待进一步研究。

Objective：To observe polygonatum polysaccharide （PSP）protective effect on LPS -induced human umbilical vein endothelial ceils （HUVEC） injury. Method ： M3T was used to detect the influence of PSP on LPS - induced HUVEC injury. Ho echst33258 was used to investigate PSP anti - apoptotic effect. Result ： PSP cultured with HUVEC did not cause obvious changes in cell viability,excluding PSP direct cytotoxicity on cells. Tested by MTT assay,HUVEC cultured with LPS and PSP can sig nificantly reduce the damage caused by LPS on HUVEC （P 〈 0.05）. It found that the nuclear of normal group was larger and u niform staining by Hoechst33258. After HUVEC cultured with LPS,the nuclear had visible stain dense granular fluorescence, showing nuclear eondensation and nuelear fragmentation eharacteristies. After giving PSP,the injury was attenuated（P 〈0. 05）. Conclusion ：PSP protect LPS -induced HUVEC injury. Anti -apoptotic role may be the mechanism of PSP protecting HUVEC.