丙酮丁醇产生菌的筛选及玉米芯水解液发酵丁醇研究

Screening of ABE producing strains and study on fermenting butanol with corn cob hydrolysate

对从自然界筛选的丙酮丁醇产生菌发酵玉米芯水磨酶水解液进行了研究。结果表明:从133份森林土样中筛选到的205株丙酮丁醇产生菌中,G-23菌株能够有效地利用葡萄糖、木糖以及葡萄糖和木糖的混合糖进行丙酮丁醇(acetone-butanol-ethanol,ABE)发酵。利用水磨的方法对玉米芯进行预处理,该方法能够有效提高玉米芯的酶水解效率,得到的水解液能直接进行丙酮丁醇发酵。利用G-23菌株对水磨预处理后的酶水解液进行ABE发酵,得到了较高产量的丁醇和总溶剂,分别为7.6g/L和12.8 g/L。

The study on ABE producing strains screened from nature to ferment the enzymatic hydrolysate of corn cob pretreated by levigation was carried out. 205 ABE producing strains were screened from 133 forest soil samples. Among them, G - 23 strain was able to use glucose, xylose and the mixture of both to ferment ABE. The results showed that corn cobs pretreated by levigation could effectively promote the enzymatic hydrolysis while the hydrolysate could be directly used in ABE fermentation. The ABE fermentation by G - 23 strain using cellulase hydrolysate pretreated by levigation was performed and then 7.6 g/L butanol and 12.8 g/L ABE were obtained.