丙泊酚或依托咪酯对大鼠空间学习记忆能力的影响

Effects of propofol or etomidate on spatial learning and memory in rats

目的观察丙泊酚或依托咪酯对大鼠空间学习记忆能力的影响。方法雄性SD大鼠45只,4周龄,随机均分为对照组、丙泊酚组和依托咪酯组。Morris水迷宫测试大鼠空间学习记忆能力,半定量逆转录-聚合酶链反应(RT-PCR)方法检测大鼠海马组织中神经球蛋白(Ngb)、碱性成纤维细胞生长因子(bFGF)和钙离子/钙调素依赖性蛋白激酶Ⅱ(CaMKⅡ)mRNA的表达。结果与第1天比较,三组大鼠第2、3、4、5天逃避潜伏期均逐渐缩短(P<0.05);与对照组比较,丙泊酚组和依托咪酯组第4、5天逃避潜伏期延长(P<0.05)。各组大鼠Ngb的表达量差异无统计学意义,而丙泊酚组和依托咪酯组bFGF和CaMKⅡ的表达量明显少于对照组(P<0.05)。结论丙泊酚或依托咪酯可使幼年大鼠的空间学习记忆能力降低,其机制可能与降低海马bFGF和CaMKⅡ的表达有关。

Objective To observe the effects of propofol or etomidate on spatial learning and memory in rats. Methods Forty-five male Sprague Dawley rats aged 4 weeks were randomly divided into three groups （n=15 each）. The rats were tested for spatial learning and memory with Morris water maze （MWM） method. Hippoeampal neuroglobin （Ngb）, basic fibroblast growth factor （bFGF） and ealeium- calmodulin-dependent protein kinase type 2 （CaMK Ⅱ） mRNA were detected by semi-quantitative reverse transcription polymerase chain reaction （RT-PCR）. Results The latency to escape from water in MWM was shortened day by day in all of groups. In 4th or 5th day, the latency in propofol group or etomidate group was longer than that in control group（P〈0. 01）. Ngb did not change while bFGF and CaMK Ⅱ mRNA were downregulated in propofol group or etomidate group. Conclusion Propofol or etomidate may impair spatial learning and memory of young rats by hippocampal bFGF and CaMK Ⅱ mRNA downregulation.