摘要
目的:采用HPLC法同时测定三果汤中具有抗氧化活性成分没食子酸和鞣花酸含量的方法。方法:采用的色谱柱为welchrom C18色谱柱(50mm×4.60mm,5μm),流动相为乙腈-0.25%甲酸溶液梯度洗脱,流速为0.85mL/min,柱温25℃,检测波长为260nm。结果:没食子酸和鞣花酸分别在0.0500～0.7000mg,0.0502～0.7140mg范围内呈良好线性,平均回收率分别为103.22%,97.49%(n=6)。结论:采所建方法适用于同时测定三果汤中没食子酸和鞣花酸的含量。
Objective：To develop RP-HPLC for the determination of antioxidant contents including gallic acid and ellagie acid in Sanguo Decoction. Methods：The separation was performed on a welchrom C18 （50mm × 4.60mm ,5 μm）column with gradient elution. The mobile phase was acetonitrile-0.25% methanoic acid solution,and the flow rate was 0.85mL/min. The detection wave- length was set at 260nm. Billand column temperature was set at 25℃. Results：The linear ranges of gallic acid and ellagic acid were within 0. 0500 - 0. 7000mg and 0. 0502 - 0. 7140mg, respectively. The average recoveries for gallic and ellagie acid were 103.22% ,97.49% （ n = 6 ）. Conclusion：This method is suitable for the determininalion of gallic acid and ellagic acid in Sanguo Decoction.
出处
《辽宁中医杂志》
CAS
2012年第12期2460-2462,共3页
Liaoning Journal of Traditional Chinese Medicine
基金
国家自然科学基金项目(30973957
30960507)
高校博士点专项科研基金项目(20095132110008)
关键词
藏药
三果汤
没食子酸
鞣花酸
含量测定
Tibetan medicine
Sanguo Decoction
gallic acid
ellagic acid
determination
