眼针对脑缺血再灌注模型大鼠COX-2、PGE_2、IL-1β表达影响的实验研究

Experimental Study on Effect of Eye-acupuncture Upon COX-2,PGE2 and IL-1β Levels of Cerebral Ischemia Reperfusion Rat Model

目的:探索脑缺血再灌注损伤后炎症反应发展变化规律,观察眼针疗法对脑缺血再灌注损伤的炎症反应影响。方法:应用线栓法复制出脑缺血再灌注损伤大鼠的模型,采用眼针治疗。再灌注24h后处死取材,采用ELISA方法对各组大鼠血清中COX-2,PGE2,IL-1β的含量进行检测。结果:眼针治疗组及体针治疗组大鼠血清中COX-2,PGE2,IL-1β含量较模型组有所降低,有统计学意义;眼针对照组与模型组比较变化不显著,没有统计学意义。结论:眼针疗法对脑缺血再灌注损伤大鼠的治疗作用可能通过其下调COX-2,PGE2,IL-1β的表达而实现。

Obiective ：To explore the rule of the inflammatory development after cerebral ischemia reperfusion injury, and observe the eye-acupuncture curative effect on the inflammatory response of ischemia reperfusion injury. Methods： The string thrombus method was used to replicate the rat model of cerebral isehemia reperfusion injury ,which used eye-acupuncture therapy. The rats were sacrificed after 24 hours＇reperfusion and ELISA method was used to detect COX-2,PGE2 and IL-1β levels in rats＇ serum. Results：COX-2, PGE2 and IL-1β levels in serum of eye-acupuncture treatment group and body-acupuncture treatment group ,were both significantly decreased. It was statistically significant compared with that in the blank control group. The levels of eye-acupuncture control group and model group did not change significantly, therefore it had no statistical significance. Conclusion ： Eye-acupuncture therapy for rats of cerebral ischemia reperfusion injury may by through reducing COX-2, PGE2 and IL-1β levels to achieve the effect.