膀胱尿路上皮癌中PTEN、MDM2的表达及其相关性研究

Expressions of PTEN and MDM2 in bladder transitional cell carcinoma and their clinical significance

目的初步探讨膀胱尿路上皮癌中PTEN、MDM2的表达及其临床意义。方法应用sP免疫组织化学染色法对80例膀胱尿路上皮癌及20例正常膀胱黏膜组织中PTEN、MDM2基因的表达进行检测，结合临床病理因素进行分析。结果FFEN在不同病理分级（G1、G2、G3）和临床分期[浅表型（Tis-T1）、浸润型（T2-T4）]的膀胱尿路上皮癌中的阳性率（86．20％、74．07％、37．50％；80．00％、46．67％）比较差异均有统计学意义（X2=15．004，P〈0．01）（X2=9．497，P〈0．01）；MDM2在不同病理分级（G1、G2、G3）和临床分期[浅表型（Tis-T1）、浸润型（T2-T4）]的膀胱尿路上皮癌中的阳性率（82．75％、55．55％、37．50％；70．00％、43．35％）比较差异均有统计学意义（x2=11．543，P〈0．01；x。=5．556，P〈0．05）；PTEN和MDM2在膀胱尿路上皮癌中的表达呈负相关（r=-0．611，P〈0．05）。结论PTEN和MDM2的异常表达在膀胱尿路上皮癌的发生发展中起重要作用，联合检测对于判断膀胱尿路上皮癌的恶性程度和预后有重要意义。

Objective To investigate the expressions of PTEN and MDM2 in bladder transitional cell carcinoma （BTCC） and their clinical significance. Methods The expressions of PTEN and MDM2 were detected by tissue immunohistochemistry test （SP method） in BTCC （ n = 80） and normal bladder tisH2O sues （ n = 20）. The relationship between PTEN and MDM2 as well as their correlations with clinical pathoH2O logical features were analyzed. Results The positive rate of PTEN in different pathological grading（ G1, G2, G3） and clinical staging [ superficial （ Tis- T1 ）, infiltration （ T2 - T4 ） ] was （ 86. 20%, 74. 07%, 37. 50% ;80. 00% ,46. 67% ）, respectively, with a significant difference （ X2 = 15. 004, P 〈 0.01 ; X2 = 9. 497, P 〈0. 01 ）. The positive rate of MDM2 in different pathological grading（ G1, G2, G3 ） and clinical staging [ superficial （ Tis- T1 ）, infiltration （ T2-T4） ] was （ 82. 75%, 55.55%, 37.50% ; 70. 00%, 43.35 % ）, respectively, with a significant difference （ X2 = 11. 543, P 〈 0.01 ; X2 = 5. 556, P 〈 0. 05 ）. The expression of PTEN was negatively correlated with that of MDM2 in BTCC （ r = H2O0. 611, P 〈 0. 05）. Conclusions Expressions of PTEN and MDM2 might be involved in the BTCC pathogenesis. The combined detection of PTEN and MDM2 might be of great value in the prediction of tumor behavior and prognosis.