基于生长代谢耦联的1,3-丙二醇发酵过程底物流加控制策略

Coupled-feeding Strategy Based on the Cell Growth and Metabolism during 1,3-Propanediol Fermentation

研究了克雷伯氏杆菌转化甘油生产1,3-丙二醇的底物流加控制策略,其特征是批式流加发酵过程与细胞生长和代谢相耦联.通过细胞生长动力学分析,建立了对数生长期和稳定期底物消耗与生物量和碱液消耗量之间的函数关系.2次在线反馈补料发酵实验结果表明,甘油浓度控制在20±2g/L时,1,3-丙二醇的终浓度分别达80.3和78.8g/L以上,与手动反馈补料相比,1,3-丙二醇浓度分别提高了25.0和23.5g/L.

A substrate feeding strategy was studied in the fed-batch fermentation of 1,3-propanediol by Klebsiella pneumoniae, which was distinctively coupled with cell growth and metabolism using glycerol as substrate. The functional relationship between substrate consumption, biomass and alkaline consumption was established by analysis of the cell growth kinetics during logarithmic growth and stationary phase, respectively. The fed-batch on-line experimental results showed that the glycerol concentration was controlled at 20＋2 g/L, and the final 1,3-propanediol concentration reached 80.3 and 78.8 g/L, which increased 25.0 and 23.5 g/L compared with that of manual feeding-back strategy.