摘要
克隆了圆红冬孢酵母R.toruloides中的ARE基因并对转录水平进行了初步分析.结果表明,克隆得到的ARE基因的DNA序列共2624bp,含6个内含子,开放阅读框2028bp,编码675个氨基酸.在限氮培养基中培养的R.toruloides酵母细胞中,ARE基因的转录表达水平随培养时间增加而提高,推测在酵母R.toruloides中ARE基因可能与油脂积累有关.
The ARE gene of Rhodosporidium toruloides was cloned and its transcriptional expression level analyzed. 2 624 bp DNA sequence of ARE gene with 6 introns was clarified. The open reading frame was 2 028 bp coding 675 amino acids. The transcriptional expression of ARE gene in the cells cultivated in nitrogen-limited medium increased with culture time. It was supposed that the transcriptional expression of ARE gene was related to lipid accumulation in R. toruloides.
出处
《过程工程学报》
CAS
CSCD
北大核心
2012年第6期1002-1007,共6页
The Chinese Journal of Process Engineering
基金
科技部国际合作基金资助项目(编号:2010DFB40170)
关键词
圆红冬孢酵母
甘油三酯
ARE基因
转录表达
Rhodosporidium toruloides
triacylglycerol
ARE gene
transcriptional expression
