摘要
报道了利用 30 mml/L HAc-NaAc为缓冲液测定复方新诺明中甲氧苄胺嘧啶(TMP)和磺胺甲基异噁唑(SMZ)的离解常数及含量的毛细管区带电泳(CZE)方法。该方法具有简便、快速、准确等特点。测得TMP和SMZ的pKa分别为6.60和5.90。其平均回收率分别为101.5%和99.6%,最低检出限为0.48 mg/L和0.028 mg/L,RSD为1.26%和1.12%。
A rapid and reliable capillary zone electrophoresis method has been established for the determination of trimethoprim and sufamethoxazole in co-trimoxazole tablets as well as their dissociation constants. With 30 mmol/L acetate as buffer electrolyte (pH = 5. 55), the two components in co- trimoxazole tablets were separated, and ethanol used as solvent in analysis procedure did not interfere with their determination. The relative standard deviation for trimethoprim and sufamethoxazole was 1 .3% and 1.1%, respectively. The detection limit was 0.48 mg/L for trimethoprim and 0. 028 mg/L for sufamethoxazole. The recovery of trimethoprim was 101. 5% and that of sufamethoxazole was 99. 6%. The contents of trimethoprim and sufamethoxazole obtained by the method were in good agreement with the nominal values. It was that the capillary electrophoresis method could be employed conveniently to determine the dissociation constants of trimethoprim and sulfamethoxazole. The dissociation constant of trimethoprim and sulfamethoxazole obtained from capillary electrophoresis method was 6. 60 and 5. 90, respectively The determined dissociation constants were closed to the values append in some common physical and chemical handbooks.
出处
《分析化学》
SCIE
EI
CAS
CSCD
北大核心
2000年第7期793-797,共5页
Chinese Journal of Analytical Chemistry
基金
国家自然科学基金!(No.2945194和29675007)
中国博士后科研基金
关键词
毛细管区带电泳
复方新诺明
TMP
SMZ
含量测定
Capillary zone electrophoresis
co-trimoxazole tablets
trimethoprim
sulfamethoxazole
dissociation constant