高效液相色谱法测定小鼠胰腺组织中头孢匹胺浓度

Determination of Cefpiramide Concentration in Pancreatic Tissue of Mice by RP-HPLC

目的建立高效液相色谱法（HPLC）测定小鼠胰腺组织中头孢匹胺浓度的方法。方法以DISCOVERY ODS C18柱（5μm，250×4．6mm）为色谱柱，甲醇：三乙胺醋酸：水（27：0．2：72．8，v/v）为流动相，流速1．0mL·min^-1，柱温30℃，紫外检测波长254nm。结果小鼠胰腺组织中的内源性物质不干扰头孢匹胺的测定，头孢匹胺在0．1～500．0μg·mL^-1浓度范围内峰面积与浓度线性关系良好（r=0．9998），最低检测限为0．1μg·mL^-1。平均回收率为（97．18±0．74）％，高、中、低3种浓度的日内差（RsD）〈5％，13间差（RSD）〈10％。结论本方法简便、快速、准确，重现性好，可应用于头孢匹胺组织内药动学研究。

Objective To estabhsh a reversed-phase HPLC（RP-HPLC） technique for the quantification of the cefpi- ramide（CPM） concentration in pancreatic tissue. Methods The following RP-HPLC conditions were set up DISCOVERY ODS C18 column（5μm, 250 mm×4.6 mm） as stationary phase; a mixture of methanol, triethanolamine and water（27-0.2-72.8） at a flow rate of 1.0 mL·min-1 as mobile phase; UV detection at 254 nm. Results CPM concentration of pancreatic tissue showed a linear plot in the range of 0.1-500.0μg·mL^-1（r=0.999 8）. The minimal quantitative concentration was 0.1μg·mL^-1. The mean recovery at the quantitative concentrations of CPM was （97.18±0.74）%. The coefficients of variation for within-day performance were found to be less than 5%, and between-day performance less than 10%. Conclusion The RP-HPLC is simple and sensitive to quantify cefpiramide concentra- tion of pancreatic tissue, and may be used to monitor the PK parameters in tissues.