摘要
应用RT-PCR和RACE技术,克隆获得了葡萄鲨烯合成酶基因(SQS)的全长cDNA(1595 bp)。序列分析结果表明:该基因包含1个完整的1242 bp开放阅读框,编码413个氨基酸残基;葡萄SQS与三七(Panax notoginseng)、人参(Panaxginseng)、甘草(Glycyrrhiza uralensis)的SQS分别有84%、83%、84%的一致性。
The full -length cDNA （1595 bp） of squalene synthase gene （SQS） in Vitis vinifera was cloned by RT -PCR and RACE here. Sequence analysis showed that this gene contained a complete open reading frame of 1242 bp encoding 413 amino acid residues. The SQS gene in Vitis vinifera showed 84%, 83%, 84% similarity with its homology in Panax notoginseng, Panax ginseng, Glycyrrhiza uralensis , respectively.
出处
《江西农业学报》
CAS
2012年第11期8-10,13,共4页
Acta Agriculturae Jiangxi
关键词
葡萄
鲨烯合成酶
基因克隆
序列分析
Vitis vinifera
Squalene synthase
Gene cloning
Sequence analysis
