固态发酵生产饲用β-葡萄糖酶的产业化技术研究

Industrial Production Technology of the β-Glucanase with Solid-state Fermentation

采用黑曲霉菌株An08-752,经曲盘、发酵床(帘子床)、厚层通风制曲池进行固态发酵,产业化生产饲用β-葡萄糖酶,结果表明:通风曲池厚层通风发酵培养30～32 h,产β-葡聚糖酶最高,发酵酶活力达到1.15×105μ/g;发酵床发酵培养时间35～37 h,产β-葡聚糖酶最高,发酵酶活力达到1.28×105μ/g;曲盘发酵培养32～34 h,产β-葡聚糖酶最高,发酵酶活力达到1.36×105μ/g.

The industrial production of β-glucanase was studied in the solid-state fermentation, by Aspergillus niger An08-752 strain with three kinds of equipments. The result showed： the β-glucanase activity reached 1.15× 10^5 μ/g dry koji at 30-32 h by thick layer ventilation koji tank in the solid-state fermentation; the β-glucanase activity reached 1.28×10^5 μ/g dry koji at 35×37 h by fermentation tank in the solid-state fermentation;the β- glncanase activity reached 1.36×10^5 μ/g dry koji at 32-34 h by koji tray in the solid-state fermentation.