发病前后应用丁苯酞对SOD1-G93A转基因鼠影响的比较

A comparison of neuroprotective effects of DL-NBP on SOD1G93A mice when treatment started at pre-symptoms or late-symptoms

目的观察丁苯酞在发病前后给药对SOD1G93A转基因鼠的影响。方法随机将40只雄性SOD1G93A转基因鼠分为4组,分别为发病前用药组、发病前安慰剂组、发病后用药组和发病后安慰剂组。根据Vercelli A评分判断发病及死亡时间。结果发病前用药具有推迟SOD1G93A转基因鼠发病时间和延长生存期的作用,与安慰剂相比,均有显著差异(P<0.05)。发病后用药能延长SOD1G93A转基因鼠生存期,与安慰剂相比,有显著差异(P<0.05)。发病前用药组的生存期与发病后用药组相比,P>0.05,无统计学差异。结论丁苯酞在发病前和发病后给药均具有延长SOD1G93A转基因鼠生存期的作用。

Objective To investigate the effects of DL-NBP on SOD1G93A mice when treatment started at pre-symptoms or late-symptoms.Method 40 Male SOD1-G93A mice were randomly divided into 4 groups,treatment of pre-symptoms,vehicle of pre-symptoms,treatment of late-symptoms and vehicle of late-symptoms.The Vercelli A score was used to assess disease onset and death of SOD1G93A mice.Results DL-NBP treatment at pre-symptoms stage could significantly delayed disease onset and prolonged disease survival of SOD1G93A mice,there was statistical difference compared with vehicle（P0.05）.DL-NBP treatment at late-symptoms stage could significantly prolonged disease survival,there was statistical difference compared with vehicle（P0.05）,However,there was no statistical significantly on extend the lifespan of SOD1-G93A transgenic mice（P0.05）.Conclusion The lifespan of SOD1G93A mice were prolonged when DL-NBP treatment started at pre-symptoms or late-symptoms.