六安瓜片茶高效液相色谱指纹图谱研究

Fingerprint of Lu’an Guapian tea based onhigh performance liquid chromatography

采用高效液相色谱(HPLC)技术对六安瓜片毛茶的指纹图谱进行了研究。结果表明,以乙腈+0.03%三氟乙酸为流动相,采用梯度洗脱的方法,毛茶的各种主要组分可得到良好的分离;通过对12个样品HPLC图谱的分析,共确定了16个色谱峰作为六安瓜片毛茶的特征指纹峰,建立了六安瓜片毛茶的HPLC指纹图谱;通过与2个霍山黄芽样品相比较,二者HPLC图谱存在较大的差异。同时通过对样品互补性系统聚类分析、主成分分析可以将不同茶类、不同产地的六安瓜片毛茶区分开来,表明得到的色谱图可以作为六安瓜片茶不同产地判别的依据,也可为六安瓜片质量控制提供实验依据。

Using high performance liquid chromatography （HPLC） technology to study the fingerpingt of Lu＇ an Guapian. The result showed that：the main component could be separated well with acetonitrile and 0.03% three trifluoroacetic acid as the mobile phase,by the gradient elution method. The 12 samples of HPLC fingerprints showed that this method could identify a total of 16 chromatographic peaks as Lu＇an Guapian tea fingerprint peaks. Then establish HPLC fingerprinting of Lu＇an Guapian tea. Compared with two Huoshan Huangya samples,the result showed that the two HPLC maps of Huangya were much diffient from Lu＇an Guanpian maps. And the different areas of Lu＇an Guapian teas HPLC chromatogram also exists some differences. The result showed the chromatogram could be used as the basis for the different origin of Guanpian tea,and also provided the experimental basis for the Lu＇an Guapian quality control.