超高压处理对牡蛎中副溶血性弧菌的消减作用研究

Study on inactivation of Vibrio parahaemolyticus in oyster by high-hydrostatic pressure

为探索有效的消减副溶血性弧菌(Vp)的超高压处理条件,利用CDC-1(临床分离株,血清型O3:K6)和CGMCC1.1997(标准菌株,血清型O1:K1)两种菌株,研究了不同Vp经超高压处理的消减变化情况;比较了CDC-1菌株接种到牡蛎体内与混合到牡蛎肉中的超高压处理的差异性。结果显示,超过200MPa的压力对两种Vp均有明显的消减作用,增大压力和延长处理时间可以提高超高压处理对Vp的消减作用;多重比较显示两种Vp对超高压的耐受力存在显著差异(p<0.05),CDC-1较CGMCC1.1997有更强的超高压耐受力;超高压处理对两种接种方式Vp的消减效果存在显著差异(p<0.05),接种到牡蛎体内的CDC-1菌株的耐受力高于混合在牡蛎肉中的CDC-1菌株;用300MPa处理10min或350MPa处理5min能有效的消减牡蛎中的Vp。

：To obtain the optimum condition of high-hydrostatic pressure on Vibrio parahaemolyticus（Vp）,two strains,CDC-1 （clinical isolates, O3：K6 serotype） and CGMCC1.1997 （reference strain,O1 ：K1 serotype） were used in this study. Their resistances to high pressure were investigated and the resistances of CDC-1 strains in live oysters and in oyster homogenates was also tested. Results showed that there was an obvious reduction of Vp in oyster homogenates at pressure levels of ≥200MPa,and HPP was proved to be an efficient means of reducing Vp in oysters, Increasing pressure and extending time could enhance inactivation of Vp. Multiple demonstrated that there were significant differences between the CDC-1 strain and the CGMCC1.1997 strain （p〈0.05）,and the CDC-1 strain had higher resistance to HPP. Resistance variations of CDC-1 strain were detected in live oysters and in oyster homogenates（p〈0.05）,the CDC-1 strain was of higher resistant to HPP in oyster. To inactivate the Vp in oyster pressure treatment needed to be conducted at pressure levels of 300MPa for 10min or 350MPa for 5min.