软枣猕猴桃根蒽醌类成分超声提取及其体外抗氧化活性研究

Study on extraction and antioxidant activity of anthra quinones from root of Actinidia arguta by ultrasonic technology

以东北地区软枣猕猴桃根为原料,以蒽醌提取率为指标,通过单因素和响应面实验对软枣猕猴桃蒽醌类化合物提取工艺中乙醇浓度、料液比、提取时间、提取温度等影响因素和提取液的抗氧化活性进行探讨。采用化学模拟体系分析了其对DPPH自由基的清除能力、对Fe3+离子的还原能力以及与Fe2+的螯合能力并与VC进行比较。结果表明超声波法提取软枣猕猴桃根蒽醌的最佳工艺:超声波率200W,乙醇浓度80%,料液比1∶25,温度70℃,提取时间15min,在此条件下软枣猕猴桃根中蒽醌类成分的提取率为1.40%;软枣猕猴桃根蒽醌类成分具有较强的清除DPPH自由基能力、还原力和螯合能力,并与质量浓度呈一定正相关关系。

Extraction technology of anthraquinones from root of Actinidia arguta was studied by using ultrasionic sound extraction equipment and taking extraction ratio of flavonoids as marker. Effects of ethanol concentration, ration of material to liquid,extracted time and extracted temperature on extraction rate of anthraquinones from root of Actinidia arguta by single factor test and response surface methodology. Its scavenging ability to DPPH free radical reduction ability to Fe3＋ and chelating ability to Fe2＋ were analyzed by chemical simulated system and compared with those of vitamin C. The optimum extraction conditions as follows.ethanol concentration 80%, ultrasionic sound power 200W,extracted time 15min,extracted temperature 70%. Under these conditions, the extraction rate was up to 1.40%. The extract obtained had a powerful scavenging activity against DPPH free radicals, reducing ferricions and stronger chelating ability in a concentration-dependent manner.