中空纤维膜非接触式萃取-反萃分离发酵液中丁酸的研究

Non-contact extraction and back extraction of butyric acid from fermentation broth by using Hollow-Fiber membrane

以丁酸发酵液为原料,采用聚偏氟乙烯PVDF中空纤维膜对发酵液中高浓度的丁酸进行了萃取-反萃研究。考察了发酵液初始pH、丁酸初始浓度、发酵液流速、有机相流速及反萃剂流速对丁酸分离效果的影响,通过响应曲面法确定了最佳工艺条件。结果表明,发酵液初始pH对丁酸分离效果影响最大,发酵液流速及丁酸初始浓度次之,萃取相及反萃剂流速对分离效果影响不大。在萃取相与反萃剂流速均为90mL/min,发酵液流速为129mL/min,发酵液中丁酸含量为74.5mg/mL,发酵液初始pH为2.2时,丁酸最高回收率为85.22%。

The extraction of butyric acid from fermentation broth across microporous hollow fibers to an organic phase and then back extraction to stripping agent was studied. Effects of various factors including the initial pH of butyric acid, the concentration of butyric acid ,the flow rate of fermentation broth ,organic phase and stripping agent on extraction yield were investigated ,and the optimum process conditions were determined by response surface methodology, Results showed that the initial pH of butyric acid had exerted a great influence on the butyric acid separation comparing with other factors. The highest recovery rate of butyric acid was 85.22% when fermentation broth was treated at an initial pH of 2.2 and butyric acid concentration of 74.5mg/mL and the flow rate of fermentation broth, the organic phase and stripping agent were 129mL/min, 90mL/min, respectively.