大剂量牛磺酸对小鼠自主活动能力的影响

Effect of large-dose taurine on locomotor activity of mice

探讨了大剂量牛磺酸对小鼠自主活动能力的影响。将8周龄雄性BALB/C小鼠分为3组:对照组、牛磺酸1组(250mg/kg·bw)、牛磺酸2组(500mg/kg·bw),每日灌喂水和相应剂量的牛磺酸。连续灌喂10d后用Smart小动物行为活动记录分析系统进行旷场和高架十字迷宫实验,用BIOSEB小鼠悬尾自动分析系统进行尾悬挂实验。旷场实验结果显示,与对照组相比较,牛磺酸1组和2组小鼠在周边、中央区域的活动性都明显提升(p<0.05),不动时间百分率都显著降低(p<0.05),慢速和快速移动时间百分率则都显著升高(p<0.05);高架十字迷宫实验结果显示,牛磺酸1组小鼠在开臂的滞留时间比对照组明显延长(p<0.05),牛磺酸2组小鼠在开臂的滞留时间和移动距离百分比都较对照组显著增加(p<0.05);尾悬挂实验结果显示,各组小鼠的活动能力等指标无明显变化。以上结果提示,大剂量牛磺酸能够提高小鼠的自主活动与探究能力,在一定程度上缓解焦虑状态,但不能缓解抑郁模型中小鼠的"行为绝望"状态。

In order to study the effect of large-dose taurine on locomotor activity of mice , 8 -weeks -old male BALB/C mice were divided into 3 groups：control group,taurine group 1 （250mg/kg. bw）,and taurine group 2 （500mg/kg .bw） based on their body weights. 10d later,open-field test,elevated plus-maze test and tail suspension trial were conducted. Open-field test results：compared with control group,taurine group 1 and 2 had more locomotive activity in both of periphery and central area（p〈0.05）,and had less immobility time（%） （p〈0.05） ,and had prolonged slow-moving and fast-moving time（%）（p〈0.05）. Elevated plus-maze test results- compared with control group,the percentage of time in open arms was markedly increased in taurine group 1 （p〈0.05）,and both of the percentage of time and moving distance in open arms were significantly improved in taurine group 2（p〈0.05）. Tail suspension trial results ：there were no differences on immobility time,and total energy and power of movement among three groups. All the above data suggested taurine improved the iocomotive activity and exploring ability of mice,and showed some anxiolytic effect but no antidepression effect.