多药耐药鲍氏不动杆菌转座子与插入序列遗传标记研究

Investigation of genetic markers of transposons and insertion sequences in multidrug-resistant Acinetobacter baumannii

目的了解医院多耐药鲍氏不动杆菌中转座子、插入序列遗传标记的存在情况。方法收集医院2008年3-11月患者标本中分离的多耐药鲍氏不动杆菌共20株,采用聚合酶链反应(PCR)的方法分析tnpU、tnp513、ISaba1等转座子、插入序列遗传标记及ISaba1-ADC连锁检测。结果 20株多耐药鲍氏不动杆菌中检出tnpU、tnp513转座子遗传标记各15株,检出率均为75.0%;ISaba1插入序列遗传标记17株,检出率为85.0%;ISaba1-ADC连锁检测17株,检出率为85.0%。结论该组多耐药鲍氏不动杆菌对β-内酰胺类、氨基糖苷类等药物呈多药耐药,可能与菌株携带转座子、插入序列等可移动遗传元件相关。

OBJECTIVE To understand the distribution of genetic markers of transposons and insertion sequences in multidrug-resistant Acinetobacter baumannii（A.baumannii） isolated from this hospital.METHODS From Mar,2008 to Nov,2008,20 strains of multidrug-resistant A.baumannii were isolated from the clinical specimens.Then,genetic markers of transposons and insertion sequences： tnpU,tnp513,ISaba1,and linkage detection of ISaba1-ADC were analyzed by PCR.RESULTS In 20 strains of multidrug-resistant A.baumannii,there were 15 strains tested with tnpU and 15 strains tested with tnp513,both of the detection rates were 75.0%.There were 17 strains tested with ISaba1,with the detection rate of 85.0%,and there were 17 strains with the linkage detection of ISaba1-ADC,with the detection rate of 85.0%.CONCLUSION The group of the multidrug-resistant A.baumannii showed multidrug-resistance to the β-lactam antibiotics and aminoglycoside antibiotics,which may be related to the transposons and insertion sequences.