临床主要革兰阴性菌耐药表型的检测与分析

Detection of drug resistance phenotypes of main clinical isolafes of gram-negative bacteria

目的研究分析医院主要革兰阴性菌的耐药表型流行现况与发展趋势。方法采用VITEK-2全自动微生物鉴定与药敏分析仪及其所携带的高级专家系统AES,WHONET5.4统计软件分析。结果革兰阴性菌占临床感染的70.0%,β-内酰胺酶耐药表型为最主要的流行表型,总检出率达92.0%;病原菌标本主要来源于痰液、尿液,占64.0%、23.0%;多集中于危急症病区。结论β-内酰胺酶耐药表型广泛流行,多种耐药表型呈现不均等分布;临床应监测细菌耐药表型,合理使用广谱抗菌药物。

OBJECTIVE To investigate the prevalence of drug resistance phenotypes of the main gram-negative bacteria and discuss the development trend.METHODS The drug susceptibility testing was performed by using VITEK 2 Compact advanced expert system（AES）,and statistical analysis was performed with WHONET 5.4.RESULTS The clinical infections caused by gram-negative bacteria accounted for 70.0%,β-lactam resistant phenotype was the most prevalent type,the total detection rate was 92.0%;the pathogens were mainly isolated from the sputum（64.0%） and urine（23.0%）;the majority of the isolates distributed in the critical emergency wards.CONCLUSION The β-lactam phenotypes have widely prevailed and various phenotypes display disproportional distribution in the wards;it is necessary for the hospital to monitor the bacterial resistance phenotype and reasonably use broad spectrum antibiotics.