高效液相色谱-激光诱导荧光-增强型电荷耦合器件检测氨基酸的研究

Studies on the Determination of Amino Acids by High Performance Liquid Chromatography with Laser Induced Fluorescence Intensified Charge Coupled Device Detector

以 3 -对羟基苯甲酰喹啉 -2 -甲醛 ( CBQCA)为检测痕量氨基酸的柱前衍生试剂 ,利用高效液相色谱 -激光诱导荧光 -增强型电荷耦合器件检测器 ( HPLC-LIF-ICCD)系统 ,考察了氨基酸衍生和 HPLC分离过程中多种因素的影响 ,建立了 HPLC-LIF-ICCD分离检测痕量氨基酸的新方法 ,并应用于鼠脑微透析液的氨基酸检测 .当信噪比为 3时 ,对甘氨酸的质量检出限为

A High performance liquid chromatography laser induced fluorescence intensified charge coupled device(HPLC LIF ICCD) system was used for the analysis of amino acid. Using 3 (4 carboxybenzoyl) 2 quinoline carboxyaldehyde(CBQCA) as a derivatizing agent, several factors influencing the derivatization and separation of amino acid were investigated in detail. The mass detection limit of glycine was 5×10 -15 mol. The method was applied to the detection of amino acids in rat brain microdialysates.