替普瑞酮对中暑大鼠多器官功能损伤保护作用

Potective effect of geranylgeranylacetone on multiple organ injury in heatstroke rats

目的观察替普瑞酮对中暑大鼠保护作用并探讨其机制。方法选择清洁级Wistar大鼠50只,随机分为5组,分别为常温对照组、中暑模型组和替普瑞酮低、中、高剂量组,将模型组和替普瑞酮组大鼠持续暴露于43℃热舱中,持续70 min,中暑后迅速取出,采血,检测大鼠血清肌酐(CR)、血清尿素氮(SUN)、谷草转氨酶(AST)、谷丙转氨酶(ALT)水平,常规苏木素-伊红染色,观察肺组织病理改变。结果中暑模型组大鼠CR、SUN、AST及ALT水平分别为(64.89±1.55)μmol/L、(8.11±0.63)mmol/L、(162.84±7.52)U/L、(62.51±3.39)U/L,明显高于常温对照组;与中暑模型组比较,高剂量替普瑞酮预处理后CR、SUN、AST及ALT水平分别降至(47.96±1.97)μmol/L、(5.16±0.34)mmol/L、(130.63±5.53)U/L、(43.76±1.76)U/L,替普瑞酮预处理组肺组织病理改变有不同程度减轻。结论替普瑞酮能减轻中暑大鼠多器官功能损害。

Objective To examine whether geranylgeranylacetone（GGA） could attenuate heat-induced multiple organ dysfunction in rats.Methods Fifty female anesthetized Wistar rats of clean degree were randomly divided into normothermic control group（NT,n=10）,heatstroke model group（HS,n=10）,low dose GGA group（GGA1,n=10）,moderate dose GGA group（GGA2,n=10）,and high dose GGA group（GGA3,n=10）.The model and GGA groups were orally administered vehicle（arabic gum） or GGA of 50 mg/kg,100 mg/kg,and 200 mg/kg,respectively,followed by the exposure to heat（43 ℃ for 70 min） before recovery at room temperature（RT,24 ℃）.The normal control group rats were treated with vehicle and were kept at RT.A DKB-501S high precision water bath and circulation chamber of water were used to establish heatstrake model.The environment temperature was kept at 22-24 ℃ and the water temperature was kept at 45.0 ℃ for more than one hour,and then the temperature in the chamber was kept at 43.0 ℃.ResultsCompared with normothermic controls,all vehicle-treated heatstroke rats displayed hepatic and renal dysfunction,including increased levels of serum urea nitrogen,creatinine,aspartate aminotransferase,and alanine aminotransferase.The heat-stress response indicators were all significantly suppressed by GGA pretreatment.The pathological changes of lungs in heatstroke rats were alos attenuated by GGA pretreatment.Conclusion GGA preconditioning attenuates heat-induced multiple organ injury in rats.