辽宁省汉城型汉坦病毒基因亚型分析

Genetic subtypes and distribution of Seoul hanta virus in Liaoning province

目的分析辽宁省汉城型汉坦病毒(HV)的基因亚型及其分布情况。方法收集辽宁省内主要肾综合征出血热(HFRS)流行地区的鼠肺和病人血清标本,采用间接免疫荧光法(IFA)检测鼠肺中HV抗原,应用HV特异性引物以RT-PCR方法扩增标本中M和S基因片段的特异性核苷酸序列,测序后与已知病毒序列进行比较分析,构建系统发生树,以明确其型别和亚型及其地理分布情况。结果辽宁省HFRS疫区褐家鼠携带的病毒均为汉城型病毒(SEOV);对鼠肺及病人携带HV的部分M片段及部分S片段进行核苷酸序列分析表明,发现SEOV的同源性较高,变异较小,稳定性较高,而且地理位置相近地区基因组核苷酸序列的同源性很高(>98.8%),具有小范围的地理聚集现象。结论辽宁省流行的SEOV均为S3亚型,基因亚型分布比较单一。

Objective To investigate the genetic subtype and distribution of Seoul hahta virus in Liaoning province.Methods Rat lung samples and serum samples of the patients were collected in major epidemic areas and hantavirus antigens in the rat lungs were detected by indirect immunofluorescence assay.Partial M and S segments were amplified by nested reverse-transcription polymerase chain reaction（RT-PCR） using hantavirus specific primer and then sequenced,analyzed and compared with known sequences.The phyologenetic trees were constructed to analyze subtypes and geographical distribution.Results The Seoul hanta virus（SEOV） were carried by Rattus norvegicus in Liaoning province.The analytic results of partial M and S segments carried by rat lungs and patients indicated that the homology and variation of SEOV were high.The homology of nucleotides sequence was above 98.8% for samples from similar geographical areas,with a high homology for adjacent geographical areas.Conclusion The popular SEOV is S3 subtypes in Liaoning province and the gene subtypes distribution is single.