食源性金黄色葡萄球菌青霉素类药物耐药基因型的分析

Genotype analysis of penicillins-resistance in foodborne Staphylococcus aureus

目的建立多重PCR方法检测青霉素酶基因和mecA基因,了解食源性金黄色葡萄球菌两种β-内酰胺类药物耐药基因的分布情况,为金黄色葡萄球菌引起食源性疾病的防治提供参考数据。方法建立多重PCR技术检测金黄色葡萄球菌青霉素酶基因、mecA基因和16S rDNA;多重PCR方法测定食品来源的171株金黄色葡萄球菌对青霉素类药物的耐药基因型。结果 165株菌携带有青霉素酶基因(96.5%),9株菌携带有mecA基因(5.3%)。结论建立的多重PCR检测方法快速、简便、准确,可满足高通量筛选菌株的需求;食源性金黄色葡萄球菌具有很高的青霉素酶基因携带率,并存在耐甲氧西林的菌株。

Objective To establish a multiplex PCR method to detect penicillinase gene,mecA gene and 16S rDNA simultaneously,and investigate the distribution of penicillinase gene,a kind of choloylglycine hydrolase,and mecA gene in foodborne Staphylococcus aureus strains.Methods The multiplex PCR method was established to detect penicillinase gene,mecA gene and 16S rDNA.Results 165 strains were detected penicillinase gene with overall positive rate 96.5%,while 9 strains were detected both mecA gene and penicillinase gene with overall positive rate 5.3%.Conclusion The multiplex PCR method which was rapid,convenient and precise,could be used for high throughput screening of penicillinase gene,mecA gene and 16S rDNA.Most strains of foodborne Staphylococcus aureus carry penicillinase gene,and a few of them are methicillin resistant.