摘要
目的观察表皮生长因子(EGF)对肝癌细胞系HepG2增殖的影响,初步探讨C-erbB2在其中的作用机制。方法以不同浓度(0、0.1、1、10、100μg.L-1)EGF作用于肝癌细胞系HepG2,采用MTT法观察EGF对HepG2增殖的影响;采用RT-PCR检测C-erbB2 mRNA的表达变化。结果 EGF能呈剂量依赖方式诱导肝癌细胞系HepG2的增殖;10μg.L-1EGF可显著增强肝癌细胞系HepG2 C-erbB2 mRNA的表达。结论 EGF可诱导肝癌细胞株HepG2的增殖,其机制可能与上调原癌基因C-erbB2的表达有关。
Objective To investigate the effect of epidermal growth factor（EGF）on the proliferation of human hepatoma HepG2 cells and its mechanism of action. Methods HepG2 cells were treated with different concentrations of EGF （0.1,1,10 and 100 μg·L^-）. The proliferation of cells was detected by MTT and the expression of C-erbB2 mRNA was measured by RT-PCR. Results EGF induced the proliferation of HepG2 cells in a dose-dependent manner. Moreover, EGF（ 10μg·L^-1）significantly prom- oted the expression of C-erbB2 mRNA in HepG2 cells. Conclusion EGF can induce the proliferation of HepG2 ceils through a mechanism involving the up-regulation of C-erbB2 expression.
出处
《实用临床医学（江西）》
CAS
2012年第10期1-3,16,共4页
Practical Clinical Medicine
基金
江西省卫生厅科技计划项目(053034)
