佐剂性关节炎大鼠心肌线粒体超微结构改变及新风胶囊对其的影响

Changes in Ultrastructure of Myocardial Mitochondria in Rats with Adjuvant Arthritis and Effect of Xinfeng Capsules on the Changes

目的观察佐剂性关节炎(adjuvant arthritis,AA)大鼠心肌线粒体超微结构的改变及新风胶囊(Xingfeng Capsule,XFC)对其的影响。方法将60只大鼠随机分成正常组、模型组、雷公藤多苷片组、甲氨喋呤组和XFC组,每组12只,分别于大鼠(正常组除外)右后足跖皮内注射弗氏完全佐剂致炎,在致炎后第19天开始给药,其中正常组和模型组均予以生理盐水,其余3组分别给予雷公藤多苷片、甲氨喋呤片、XFC,在给药第30天用电镜观察每组大鼠的心肌线粒体超微结构的变化。结果与正常组相比,模型组大鼠心肌线粒体部分膜融合,增生肿胀,嵴稀疏,呈空泡化,模糊不清,横纹消失;与模型组相比,各治疗组大鼠心肌线粒体肿胀较轻,部分呈空泡化,嵴尚密集,略模糊,横纹较清晰。与XFC组相比,甲氨喋呤组大鼠线粒体呈圆形或椭圆形,排列在肌丝之间,线粒体肿胀及空泡化明显,嵴排列紊乱,横纹不清,而雷公藤多苷片组大鼠线粒体呈圆形或椭圆形,排列在肌丝之间,膜略欠清晰,部分线粒体肿胀及空泡化较明显,嵴呈纵行排列,略稀疏紊乱,横纹欠清楚。结论 AA大鼠存在心肌线粒体的损伤,而中药XFC能改善这种损伤。

Objective To observe the changes in the ultrastructure of myocardial mitochondria in rats with adjuvant arthritis （AA） and the effects of Xinfeng capsules （XFCs） on the changes. Methods A total of 60 rats were randomly and equally divided into normal control （NC） group, model control （MC） group, tripterygium glycoside tablet （TGT） group, methotrexate tablet （MTXT） group, and XFC group. Each rat （except that in the NC group） was intradermally injected with Freund＇s complete adjuvant in the right hind paw to induce AA. Nineteen days later, physiological saline was given to the NC group and MC group, and TGTs, MTXTs, and XFCs were given to the remaining groups. The changes in the ultrastruc ture of myocardial mitochondria in rats with AA in each group were observed under electronic microscope on day 30 of treatment. Results Compared with the NC group, the MC group showed partial membrane fusion, proliferation and swelling, sparse cristae, vacuolization, vagueness, and band disappearance in the myocardial mitochondria. Compared with the MC group, the treatment groups showed less swelling, partial vacuolization, dense cristae, slight vagueness, and clearer bands in the myocardial mitochondria. Compared with the XFC group, the MTXT group had round or oval mitochondria arranged among myofilaments, which demonstrated apparent swelling and vacuolization, disordered cristae, and unclear bands, while the TGT group had round or oval mitochondria arranged among myofilaments, which demonstrated slightly unclear membranes, apparent swelling and vacuolization （in some mitochondria）, longitudinally arranged and slightly sparse and disordered cristae, and unclear bands. Conclusion There is damage of myocardial mitochondria in AA rats, and XFCs can reverse the damage.