毛细胞白血病的超微结构诊断特征

Characteristics of Uitrastructural Diagnosis of Hairy Cell Leukamia

目的探讨毛细胞白血病（HCL）超微结构诊断特征。方法利用透射电镜观察7例HCL患者外周血或骨髓中毛细胞的超微结构特征。结果电镜下可见毛细胞，其超微结构特点是：①微绒毛特点：数量多、微绒毛长、粗细相同、形态各样；②胞核特点：卵圆形，稍有刻痕，异染色质沿核膜内面分布；③胞质特点：细胞器丰富，首次发现髓鞘结构小体，但未见核糖体板层复合体。结论毛细胞的微绒毛特点是HCL超微结构诊断的重要特征，胞核和胞质特点可用做HCL鉴别诊断的参考特征，核糖体板层复合体不一定是HCL的诊断特征。

Objective To inquire into the characteristics of ultrastructural diagnosis of hairy cell leukamia (HCL). Methods Ultrastructural characteristics of hairy cell in the peripheral blood or bone marrow from 7 cases with HCL were observed by transmission electron microscope (TEM). Results Hairy cell were seen under TEM. Its features included:①The featare of microvilli. There were numerous and long micmrovilli in surface of hairy cell. These Microvilli had same size in diameter and various shapes.②The feature of nucleus. The nucleus was oval and was lightly indented, and heterochromatins were distributed in inner surface of nuclear membrance.③The feaure of cytoplasm. There were rich organelles in cytoplasm. Ribosome-lamella complexes (R-LC) were not seen, but myelin figure body was firstly seen. Conclusion The features of microvilli of hairy cell are important characteristics of ultrastructural diagnosis of HLC. The features of nucleus and cytoplasm were reference characteristics of differential diagnosis of HCL.But R-LC was not certain for characteristics of diagnosis of HCL.