PCR检测腹膜透出液中的金黄色葡萄球菌

Detection of staphylococcus aureus in peritoneal dialysate by PCR MIAO

目的检测腹膜透出液中的金黄色葡萄球菌。方法应用PCR检测未感染的及已感染的腹膜透出液，并与传统培养方法进行对比。结果培养出金黄色葡萄球菌的腹透液，PCR均出现了279bp的特异性扩增带，且PCR检出率高于培养法。结论PCR与培养方法相比具有快速、特异、敏感的优点，而且不受抗菌素使用的限制。

Objective To detect staphylococcus aureus (SA) in peritoneal dialysate. Methods PCR was performd to detectSA in uninfected and infected specimens and compared with routine culture Results Specific amplification zone 279bp appeared with the Peritoneal dialysates which gained positive SA culture. PCR could detect more infected specimens than culture. Conclusion Compatal with culture, the method is rapid, specific, sensitive and it is not limited by ongoing antimicrobiol therapy.