薄层扫描法测定益胆片中氯原酸的含量

Determination of chlorogenic acid in Yidan tablets by TLC scanning

目的 研究中药益胆片中绿原酸的定量方法。方法 样品用甲醇提取 ,蒸干甲醇后加水使溶解 ,氯仿脱脂 ,用 10 %枸橼酸调pH1.5～ 2 .0 ,用醋酸乙酯提取 ,点样于硅胶G薄层板上 ,以醋酸乙酯 丙酮 甲酸 水 (5∶1∶1∶1)展开 ,用CS 930溥层扫描仪在λs=330nm ,λR=2 10nm下扫描测定。结果 本法的平均回收率为 95 .97% ,RSD为 2 .77%。结论 结果显示该方法灵敏、准确、专属性强 。

OBJECTIVE To study a method for the determination of chlorogenic acid in Yidan tablets.METHODS The tablets were extracted with methyl.The extract was evaporated to dry,and then dissolved in water.The water solution was defatted with chloroform and adjusted to pH1.5～2.0 with 10% citric acid,and then extracted with ethyl acetate.The sample was applied on a silica gel G plate and developed at room temperature.With ethylacetate acetone formicacid water (5∶1∶1∶1),the seperated spots were scanned by CS 930 at λ s=330 nm,λ R=210 nm.RESULTS The analytical recovery was 95.97% and RSD was 2.77%.CONCLUSION The results showed that this assay was sensitive,accurate and specific.