益龄精口服液澄清工艺优选

Optimization of Clarification Technology of Yilingjing Oral Liquid

目的:优选壳聚糖用于益龄精口服液的絮凝澄清工艺条件。方法:以吸光度、二苯乙烯苷保留率为指标,考察提取液质量浓度、壳聚糖用量、pH、温度、搅拌速度、搅拌时间等因素对絮凝效果的影响;在单因素试验基础上,采用正交试验优选提取液质量浓度、壳聚糖用量、絮凝温度等因素对絮凝工艺的影响;比较絮凝澄清法与醇沉法。结果:最佳澄清工艺条件为提取液质量浓度0.25 g.mL-1,壳聚糖用量1.2 mL.g-1,絮凝温度60℃,pH 5.0～5.5,搅拌速度300 r.min-1,搅拌时间10min。结论:壳聚糖絮凝澄清法可替代乙醇沉淀法用于益龄精口服液的澄清工艺。

Objective： To optimize flocculation clarification technology conditions of Yilingjing oral liquid by chitosan. Method： Taking absorbance and retention rate of stilbene glucoside as evaluation indicators, influence of the mass concentration of extraction liquid, chitosan dosage, pH, temperature, stirring speed, stirring time and other factors on flocculation effect were investigated. Based on single factor test, orthogonal design was selected to optimize flocculation technology with the mass concentration of extraction liquid, chitosan dosage, flocculation temperature as factors. Flocculation clarification method and alcohol sedimentation were compared. Result： Optimal clarification procedure condition was that the concentration of materials-extraction solution O. 25g·mL^-1, the adding dosage of chitosan of 1.2 mL ·g^-1, flocculation temperature of 60 ℃ , pH of 5.0-5.5, stirring speed of 300 r ·min^-1 and stirring time of 10 min. Conclusion： Chitosan flocculation method could be used as clarification technology of Yilingjing oral liquid, which could substitute ethanol precipitation method.