核桃楸不同药用部位总黄酮含量测定及变化规律

The Determination and Accumulation of Flavone in Different Medicinal Parts of Juglans mandshurica

目的:建立核桃楸药用部位总黄酮成分含量测定方法,明确其变化规律。方法:采用75%乙醇回流对青龙衣中总黄酮类成分进行提取,以芦丁为对照品,采用硝酸铝-亚硝酸钠比色法,AB-8大孔树脂除色素,建立含量测定方法。结果:初伏前后5个采集时间青龙衣总黄酮含量分别为3.15,3.49,3.90,3.98,3.96 mg.g-1。结论:初伏前黄酮总含量较低,初伏后总黄酮含量呈逐步增加的趋势,并且有一个快速增长的过程,伏天过后含量略有下降;不同药用部位黄酮含量顺序为叶>皮>茎。

Objective： To establish the determination method and variation of medicinal parts of Juglans mandshurica. Method： Extract the total flavone from Qinglongyi by 75% ethanol, take the aluminum nitrate- nitrite colorimetric, in addition to the pigment by AB-8 macroporous resin and establish the determination method. Result： The content of total flavone in five different acquisition time was 3.25, 3.49, 3.90, 3.98, 3.96 mg · g^-1. Conclusion： Before the canicular days, the content of total flavones was gradually increased; after the canicular days, the content of total flavones was significantly growth to flatten, the content of total flavones of medicinal parts of J. mandshurica was leaf 〉 bark 〉 stem.