HPLC测定樟木中黄樟醚二醇的含量

Determination of Safrolglycol in Cinnamomum camphora by HPLC

目的:建立樟木中主要成分黄樟醚二醇的含量测定方法。方法:采用C18(4.6 mm×200 mm,5μm)色谱柱;流动相乙腈-水(14∶86),检测波长282 nm,柱温30℃。结果:该成分在进样量1.014～5.07μg与峰面积线性关系良好(r=0.999 9),平均回收率为99.0%,1.89%(n=6)。结论:该方法测定樟木中主要成分黄樟醚二醇的含量,方便、准确、灵敏度高,重复性好,可适用于樟木药材的质量控制。

Objective： To establish a method for the determination of safrolglycol in Cinnamomum camphora. Method： Using HPLC with Hypersil ODS column, CH3CN-H2O （14：86） as mobile phase and detection wavelength at 282 nm. Result ： The linearity of the calibration curve was evaluated over the amount range of 1. 014-5.07 μg for safrolglycol, and good linearity with correlation coefficient （r） of 0, 999 9 was obtained. The recovery and RSD were 99.0% and 1.89% （n =6） respectively. Conclusion： This method is rapid, simple and accurate, thus may be used for the quality control of C. camphora.