HPLC测定乐脉片中丹酚酸B的含量

Determination of Salvianolic Acid B in Lemai Tablets by HPLC

目的:建立HPLC测定乐脉片中丹酚酸B含量的方法。方法:采用Waters SymmetryShield RP18色谱柱(3.9 mm×150 mm,5μm),流动相乙腈-甲醇-1.7%甲酸水溶液(4∶27∶69),检测波长286 nm。结果:丹酚酸B在0.20～2.5μg成良好的线性关系(r=0.999 9),平均回收率为100.2%(n=6,RSD 2.0%)。结论:方法简便、快速准确、灵敏度高,重复性好,可作为乐脉片中丹酚酸B的含量测定。

Objective： To establish a method for determination of salvianolic acid B in Lemai tablets by HPLC. Method： A Waters SymmetryShield RP18 column （3.9 mm× 150 mm, 5 μm） was used with the mobile phase of acetonitrile-methnol-1.7% formic acid （4： 27： 69） at the detection wavelength of 286 nm. Result： The calibration curve was linear in the range of 0.20-2.5 μg （r = 0. 999 9）. The average recovery was 100.2% （ n = 6, RSD 2.0% ）. Conclusion ： The method is convenient, quick, accurate and suitable for the determination of salvianolic acid B in Lemai tablets.