摘要
目的:研究五子衍宗方对实验性隐睾小鼠生精功能的保护作用。方法:取5~6周龄BALB/c小鼠,手术建立小鼠隐睾模型,分为假手术组,模型组,五子衍宗方低、高剂量(100,200 mg.kg-1)组,手术14 d后给药,用药14 d,于末次给药24h后检测小鼠的睾丸质量、精子密度、活率,光、电镜观察睾丸组织学结构的变化,检测小鼠睾丸内谷胱甘肽过氧化物酶(GSH-Px)、超氧化物歧化酶(SOD)活性及丙二醛(MDA)含量的变化。结果:五子衍宗方能够显著增加模型小鼠的睾丸质量(P<0.01),明显增加精子密度(P<0.05),提高精子活率(P<0.01),改善隐睾小鼠睾丸组织的病理性变化,显著升高SOD和GSH-Px活性(P<0.05或P<0.01),显著降低MDA含量(P<0.01)。结论:五子衍宗方提高睾丸组织内抗氧化损伤能力,从而减轻隐睾对小鼠睾丸生殖功能的损害。
Objective: To investigate the protective effect of Wuzi Yanzong decoction on experimental cryptorchidism mice. Method: Five-six weeks old BALB/c mice cryptorchidism was induced and divied into four groups as sham, model, low dose of Wuzi Yanzong decoction (100 mg·kg^-1), high dose of Wuzi Yanzong decoction (200 mg·kg^-1 ). At the end of the study, testis tissues were collected for detection of testes weight, sperm density, live rate, histological structure changes of testis, detection intestes glutathione peroxidase (GSH- Px), superoxide dismutase (SOD) activity and the malonyldialdehyde (MDA) content changes. Result: The Wuzi Yanzong decoction treated mice in the cryptorchid group significantly increased the mice testis weight (P 〈0.01 ) , sperm density (P 〈 0.05) , sperm live rate (P 〈 0. 01 ) and showed an improved histologic appearance. Wuzi Yanzong decoction treatment significantly increased the reduced SOD and GSH-Px enzyme activities (P 〈 0.05 or P 〈0. 01 ) and decreased the elevated tissue malondialdehyde (MDA) levels (P 〈 0, 01 ) in testis tissues samples. Conclusion: Wuzi Yanzong decoction is a potential beneficial agent to reduce testicular damage in cryptorchid mice by decreasing oxidative stress.
出处
《中国实验方剂学杂志》
CAS
北大核心
2012年第24期201-204,共4页
Chinese Journal of Experimental Traditional Medical Formulae
基金
国家自然科学基金项目(30973928)
关键词
五子衍宗方
隐睾
自由基
生精障碍
Wuzi Yanzong decoction
free radical
cryptorchidlsm
dyszoospermia